Gas exchange capacity depends on the Kv value of the corresponding filter types.
Kv is the volumetric gas exchange coefficient by means of diffusion throughout the filters. It represents the number of gas replacements in the vessels per time unit (unit: GE/day). The Kv is determined by the type of filter, filter length and gas volume in the containers. The measurements were obtained with empty vessels in standard conditions, hence these values are not indicative of the real behavior of a plantlet under specific growing conditions.
Gas exchange is achieved by diffusion throughout a series of depth filters. These conditions ensure optimal functioning:
Your choice will depend on a number of parameters, such as:
As a rule, L, XL and XXL filters are designed for plants with a long gereration time, whereas the XXL+ filters are well suited for plants in need of a high gas exchange and/or plants that spend less time in the Microbox.
Comparative in-situ tests are necessary to decide which filter type is appropriate.
In an autoclave based system, the Microboxes should not be sterilized by means of gamma irradiation.
These boxes are re-usable 7 to 10 times if treated with care.
In a fully automated and sterile system, the Microboxes should be sterilized by means of gamma irradiation.
These boxes are not re-usable. The irradiation and sterile packing take place immediately after the production, prior to shipment.
Autoclave containers and medium separately, so PP-containers hold their shape and can be re-used a number of times.
Pack stacked containers and covers separately in an autoclavable bag
Autoclave containers and covers
Unwrap containers and covers in sterile conditions
Fill containers with warm sterilized medium under LAF (Laminar Air Flow)
Carefully snap cover around entire rim to securely close
Store containers with medium in a clean area until time of use
Autoclave containers filled with medium
Fill the containers with medium
Place a piece of non-woven tissue on one edge of each container before closing the lids, to allow vapor to enter the Microbox during autoclaving
If condensation is a problem, cover lids loosely with aluminum foil to prevent filters from getting soaked
Put containers in autoclave
Slowly build up pressure to prevent lids from closing
After sterilization, slowly reduce the pressure in the autoclave. As soon as the containers are removed from the autoclave, remove the non-woven tissue and snap to close each cover completely
Store containers with medium in a clean area until time of use .
Corner of lid left open: no deformation
gas has free passage
quick pressure increase
quick pressure reduction
Hermetically-sealed lid: containers distort with pressure changes
Microbox filters are based on a depth-filtration principle—as opposed to membranes, which are surface filters. Depth filters limit dehydration: the longer the filter plug, the less dehydration. Our white filter strips have the longest filter plugs, the green filters the shortest (thus, the white filter strips limit de-hydration the most). Another factor influencing dehydration is growing medium volume.
No: thanks to an adequate gas exchange, the plantlets have been well prepared for their autotrophic life during their stay in the vessels, so weaning is no problem.
This innovative filtration technology combined with a polypropylene plastic container results in a new generation of autoclavable plant micropropagation vessels with filter: Barrier against pests and contaminations, high gas exchange, no dehydration and sterile: everything your invitro plants need for healthy development. Located in Belgium, in the centre of Europe, the Microboxes are easily shipped worldwide.
Subscribe to our mailing list
office address: Veldeken 38 b - 9850 - Nevele - Belgium - phone: +32 9 280 09 80